RNAseek

RNAseek is a powerful, all-in-one platform designed for transcriptome measurement and comprehensive RNA-seq analysis, featuring an automated pipeline that streamlines every step from raw data to biological insights.

1. Quality control with FastQC and trimming with Trimmomatic

2. Alignment with HISAT2 and BAM generation via SAMtools

3. Quantification with featureCounts for count matrices

4. Differential expression analysis with DESeq2

5. GSEA pathway and functional enrichment with gseapy

6. Visualization tools for figures: HeatMapper & PCA

Choose Your Analysis Workflow

Upload FASTQ Files

Complete RNA-seq pipeline from raw reads: QC, alignment, quantification, and differential expression analysis.

Upload Counts CSV

Skip to differential expression analysis with your own count matrix.

Upload FASTQ Files

Project Name

Genome of Interest

Sequencing Type

Single-End

Paired-End

P-value Cutoff

Upload Files

Click to upload or drag and drop

FASTQ or FASTQ.GZ files, up to 70GB each

RNAseek

Choose Your Analysis Workflow

Upload FASTQ Files

Upload Counts CSV

Upload FASTQ Files

DESeq2 Metadata

Upload Counts CSV

Sample Metadata