Welcome to

RNAseek — Bulk transcriptome measurement

12 analytical modules · 3 advanced spokes

How It Works

Three streamlined steps from raw data to actionable biological insights.

1

Upload & Configure

Bring your raw FASTQs, pre-aligned reads, or count matrices. Our high-speed chunked uploader securely transfers massive datasets directly to our computational fleet. Select a reference genome and map your metadata.

2

Automated Processing

Our Master Router dynamically executes the correct biological track (e.g., standard RNA-seq, small RNA, or epigenomics). The pipeline handles alignment, batch correction (ComBat-seq), and statistical normalization (DESeq2) automatically.

3

The CoreHub

Enter your isolated, secure workspace. Instantly explore interactive PCA and Volcano plots, download standardized matrices, and seamlessly trigger our 12 advanced analytical modules or single-cell deconvolutions right from the dashboard.

Platform Capabilities

From raw reads to spatial maps, every analysis module at your fingertips.

Alignment & QC

FastQC, Trimmomatic, HISAT2 alignment, featureCounts quantification, and compressed CRAM output.

DEG & Statistics

Batch correction (ComBat-seq), DESeq2 differential expression, PCA, UMAP, and Volcano plots.

Cell Deconvolution

DestVI / BayesPrism unmixes bulk RNA into pseudo-single-cell profiles for advanced downstream analysis.

Spatial Mapping

Tangram deep learning projects imputed cells onto H&E tissue slides with Moran’s I autocorrelation.

12 Standard Analytical Modules

Unlocked instantly after the core pipeline finishes.

Alternative Splicing

IsoformSwitchAnalyzeR detects transcript structure changes and lost protein domains.

RNA Editing & SNPs

REDItools2 scans for A-to-I editing events and high-confidence mutations.

WGCNA

Co-expression network analysis correlating gene modules to clinical traits.

Pathway Enrichment

GSEA/ORA with PathBank, KEGG, and Reactome pathway diagrams.

Causal Networks

GRNBoost2 regulatory inference and STRING protein-protein interaction maps.

Literature Mining

INDRA NLP reconstructs gene regulatory pathways from PubMed abstracts.

Survival Prediction

Kaplan-Meier curves and Log-Rank tests on gene expression cohorts.

TCGA Integration

Compare your data against massive public cancer cohorts for subtype classification.

Biomarker Discovery

Cross-reference DEGs against FDA-approved diagnostic and predictive biomarkers.

MOFA

Multi-omics factor analysis integrating RNA-seq with secondary omics data.

DIABLO

Supervised multi-omics integration for predictive modeling with AUROC curves.

Trajectory Inference

PAGA / Pseudotime mapping of pseudo-cell developmental lineages.